微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pUXV5 |
|---|---|
| 商品货号： | TS183205 |
| Designations： | pUXV5 |
| Depositors： | JA Bruenn |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Size (kb): 6.30
Vector: pUXV5 (plasmid)
Promoters: Promoter gap
Construction: pUXV1, S. hindustanus ble
Marker(s): ampR,bleR
Construct size (kb): 6.30
Features: marker(s): ampR, bleR promoter: gap replicon: pMB1, UARS |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--6.5; SacI--6.5; BamHI--6.5. Available for research purposes only. One of a series of vectors (ATCC 77463-77465 and 77468-77471) differing only in the selectable marker and the orientation of the gap promoter. Differs from pUXV6 (ATCC 77469) only in the orientation of the gap promoter and the available cloning site. Differs from pUXV7 (ATCC 77470) only in the source of the bleomycin resistance gene. This comes from Streptoalloteichus hindustanus. Shuttle expression vector using Ustilago maydis promoters gap (glyceraldehyde phosphate dehydrogenase) and hsp70 to drive expression of the cloned insert and bleomycin resistance genes respectively. The order of the major features in the plasmid is: Ustilago maydis ARS - ampR - pMB1 ori - 5 lacZ fragment - EcoRI - SacI - gap promoter -> BamHI - HindIII - hsp70 promoter -> S. hindustanus bleR -> hsp70 term - HindIII - 3 lacZ fragment. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Kinal H, et al. A family of Ustilago maydis expression vectors: new selectable markers and promoters. Gene 127: 151-152, 1993. PubMed: 8486284 Kinal H, et al. An expression vector for the phytopathogenic fungus, Ustilago maydis. Gene 98: 129-134, 1991. PubMed: 2013404 |
| Shipped： | freeze-dried |