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| 产品名称： | F5.53 |
|---|---|
| 商品货号： | TS183227 |
| Designations： | F5.53 |
| Species： | Homo sapiens, human |
| Depositors： | MI Skraastad |
| Vector： | Construct size (kb): 9.0 |
| Insert： | DNA: genomic Insert lengths(kb): 2.799999952316284 Tissue: lymphoblast GM1416 (48,XXXX) cell line Gene product: DNA Segment, single copy D4S10 Alleles: K2, A1, A2, J2, K1, L1, L2, J1 |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--5.9, 3.0; EcoRI--9.0; HindIII--9.4; BamHI--9.4; PstI--6.3, 1.25, 1.05, 0.92. The GM1416 cell line was incorrectly described as 49,XXXXY. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The distance between the EcoRI (F5.53, TS183227) and the MspI (H5.52, ATCC 61106) is 48 kb. This probe was derived from C5.5 by digesting with HindIII and religating. This is from the more centromeric end of C5.5. This probe was derived from the cosmid C5.5. C5.5 was isolated from a library constructed by digesting the vector C2RB with BamHI and SmaI and inserting size-selected fragments from GM1416 partially digested with MboI. |
| References： | Skraastad MI, et al. Mapping of recombinants near the Huntington disease locus by using G8 (D4S10) and newly isolated markers in the D4S10 region. Am. J. Hum. Genet. 44: 560-566, 1989. PubMed: 2564732 Bakker E, et al. Two additional RFLPs at the D4S10 locus, useful for Huntingtons disease (HD)-family studies. Nucleic Acids Res. 15: 9100, 1987. PubMed: 2891108 |