宁波泰斯拓生物

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pTHH59 [RW-24]

货号 TS183266
中文名称 null
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产品名称: pTHH59 RW-24
商品货号: TS183266
Designations: pTHH59 RW-24
Species: Homo sapiens, human
Vector:
Construct size (kb): 8.199999809265137
Insert:
DNA: genomic
Insert lengths(kb): 3.799999952316284
Gene product: DNA Segment, single copy D17S4
Insert Size (kb): 3.800
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 3.8; EcoRI--4.4, 3.8; HindIII--6.6, 1.7; BamHI--7.0, 1.2; HincII--3.8, 3.3, 1.15.
The probe detects a genomic EcoRI fragment of 8.6 kb and does not hybridize to rat or mouse sequences.
RFLPs detected at normal hybridization and stringency; washes at 0.1X SSC, 0.1% SDS, 65C.
Enzyme(s) not detecting polymorphism: BglII.
References:

Leach RJ, et al. Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. Genomics 5: 167-176, 1989. PubMed: 2571568

Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050

Barker D, et al. Gene for von Recklinghausen neurofibromatosis is in the pericentromeric region of chromosome 17. Science 236: 1100-1102, 1987. PubMed: 3107130

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pTHH59) on chromosome 17 D17S4. Nucleic Acids Res. 16: 3598, 1988. PubMed: 2897667

Peter J OConnell, personal communication