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| 产品名称： | pGL101 |
|---|---|
| 商品货号： | TS183268 |
| Designations： | pGL101 |
| Depositors： | G Lauer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.2000000476837160 Vector: pGL101 (plasmid) Promoters: Promoter lac Construction: pBR322, Escherichia coli lac Marker(s):ampR Construct size (kb): 2.200000047683716 Features: marker(s): ampR promoter: lac replicon: pMB1 enhancer: none |
| Applications： | contains easily purifiable cassette(s) for construction |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--2.2; EcoRI/PstI-- 1.5, 0.7. Source of Escherichia coli lacZ promoter and ribosome binding site for use in constructing expression vectors. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lauer G, et al. Construction of overproducers of the bacteriophage 434 repressor and cro proteins. J. Mol. Appl. Genet. 1: 139-147, 1981. PubMed: 6286819 |
| Shipped： | freeze-dried |