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| 产品名称： | pCMVtkLUC+ |
|---|---|
| 商品货号： | TS183313 |
| Designations： | pCMVtkLUC+ |
| Depositors： | J Duschl, J Altschmied |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.3759999275207520 Vector: pCMVtkLUC+ (plasmid) Promoters: Promoter TK Marker(s):ampR Construct size (kb): 5.375999927520752 Features: marker(s): ampR promoter: TK replicon: pMB1 reporter group: luciferase (luc+) MCS: ClaI...SacI MCS: HindIII, SalI terminator: SV40 large T-antigen polyadenylation terminator: SV40 late polyadenylation enhancer: CMV |
| Applications： | reporter construct
xa0shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.8, 0.6; EcoRI--3.2, 2.2. The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements. The vector contains the human cytomegalovirus (CMV) enhancer upstream of the tk promoter. This combination confers strong transcriptional activation not only in a variety of vertebrate cell lines, but also in vivo in whole fish embryos injected with the vector DNA. The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214 |
| Related Products： | component of:ATCC 87654 |
| Shipped： | frozen |