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微信小章| 产品名称： | HBE135-E6E7 |
|---|---|
| 商品货号： | TS183352 |
| Organism： | Homo sapiens, human |
| Tissue： | lung/bronchus |
| Cell Type： | epithelial HPV-16 E6/E7 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain HPV-16 E6/E7 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lung cancer |
| Age： | 54 years |
| Gender： | male |
| Applications： | This cell line may be used as a reference in cDNA microarray studies to demonstrate lung cancer profiles correlating with clinical outcome or biology of tumors.xa0Ref   Wigle DA, et al. Molecular profiling of non-small cell lung cancer and correlation with disease-free survival. Cancer Res. 62: 3005-3008, 2002. PubMed: 12036904xa0 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The HBE135-E6E7 cell line was derived from normal bronchial epithelium taken from a man undergoing lobectomy for squamous cell carcinoma. Cells from the primary explant in their first passage were infected with the recombinant retrovirus LXSN16E6E7 containing the human papilloma virus (HPV) E6E7 gene. Cells were selected in the presence of 0.4 mg/mL G418. Ref Tsao MS, et al. Autocrine growth loop of the epidermal growth factor receptor in normal and immortalized human bronchial epithelial cells. Exp. Cell Res. 223: 268-273, 1996. PubMed: 8601403 |
| Clinical Data： | 54 years
male |
| Comments： | Northern blot hybridization shows that immortalized HBE cells express high levels of mRNA for epidermal growth factor receptor (EGFR), transforming growth factor-alpha (TGF-alpha), and amphiregulin (AR), but not epidermal growth factor (EGF). Ref xa0 xa0 |
| Complete Growth Medium： | Keratinocyte-Serum Free medium with 5 ng/ml human recombinant EGF (do not filter) and 0.05 mg/ml bovine pituitary extract (Invitrogen, formerly GIBCO-BRL, Cat. No. 17005-042) and supplemented with 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:4 Medium Renewal: Every 2 to 3 days. xa0
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time： | 24 to 30 |
| Name of Depositor： | M Tsao |
| Deposited As： | human |
| Passage History： | Cells from the primary explant in their first passage were infected with the recombinant retrovirus LXSN16E6E7 containing the human papilloma virus (HPV) E6E7 gene. |
| Year of Origin： | 1994 |
| References： | Tsao MS, et al. Autocrine growth loop of the epidermal growth factor receptor in normal and immortalized human bronchial epithelial cells. Exp. Cell Res. 223: 268-273, 1996. PubMed: 8601403 The cell line was established from normal bronchus tissue taken at lobectomy for squamous cell carcinoma. The tissue used was at least 3 cm from the edge of the tumor. Wigle DA, et al. Molecular profiling of non-small cell lung cancer and correlation with disease-free survival. Cancer Res. 62: 3005-3008, 2002. PubMed: 12036904 |