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微信小章| 产品名称： | Human parainfluenza virus 4b |
|---|---|
| 商品货号： | TS183376 |
| Classification： | Paramyxoviridae, Paramyxovirinae, Rubulavirus, Human parainfluenza virus 4 |
| Deposited As： | Parainfluenza 4b |
| Agent： | Human parainfluenza virus 4b |
| Strain： | CH 19503 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Deposited as Parainfluenza 4b |
| Effect on Host： | CPE, cell degeneration, clumping and sloughing |
| Recommended Host： | NCI-H292 (ATCC® CRL-1848™) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously.xa0 End adsorption by adding virus growth medium. Incubation: 6-11 days |
| Name of Depositor： | JC Hierholzer, Centers for Disease Control and Prevention |
| Source： | Child with respiratory tract infection, Washington, DC, 1962 |
| References： | Canchola J, et al. Antigenic variation among newly isolated strains of Parainfluenza type 4 virus. Am. J. Hyg. 79: 357-364, 1964. PubMed: 14159955 Castells E, et al. NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses. Arch. Virol. 115: 277-288, 1990. PubMed: 22609024 |