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| 产品名称： | pJK148 |
|---|---|
| 商品货号： | TS183396 |
| Designations： | pJK148 |
| Depositors： | JB Keeney |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.3439998626708980 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pJK142 Intact vector size: 3.272 Type of vector: plasmid Vector end: NdeI Vector end: NdeI Cloning sites: SacI SacII SpeI XbaI NotI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI HincII XhoI ApaI DraII KpnI Polylinker sites: SacI SacII SpeI XbaI NotI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI HincII XhoI ApaI DraII KpnI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lac marker(s): ampR promoter: lac replicon: pMB1 Cross references: Vector: pJK148 (plasmid) Promoters: Promoter lac Construction: pJK142 Marker(s):ampR,leu1 Construct size (kb): 5.343999862670898 Features: insert detection: lacZ marker(s): ampR marker(s): leu1+ promoter: lac replicon: pMB1 |
| Applications： | contains sequence integrating vector shuttle vector vector permitting positive selection for integration vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; PstI--5.2; HindIII--2.9, 2.4. Shuttle vector for integrating sequences at leu1. Permits visual detection of recombinants by lacZalpha complementation. Constructed from pJK142 (ATCC 86955) by inserting the leu1 gene at the NdeI site. The leu1 gene contains the following unique restriction sites: StyI EcoNI Eco47III BsmI Bsu36I SplI Tth3I NdeI NruI SnaBI. The order of the major feature in the plasmid is: ampR - pMB1 ori - 5 lacZ - SacI/MCS/KpnI - 3 lacZ - leu1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| References： | Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439 Jill B Keeney, personal communication |
| Related Products： | component of:ATCC 86958 component of:ATCC 87686 |
| Shipped： | dried |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |