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微信小章| 产品名称： | Clostridium perfringens (Veillon and Zuber) Hauduroy et al. |
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| 商品货号： | TS183397 |
| Strain Designations： | Strain 34. Type b A.J. Wilsdon type B, strain 34, L.S. McClung 1955, NCIB 10691 |
| Isolation： | Intestinal contents of lamb |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | The presence of cpa, cpb, etx, cpb2, and pfoA genes was confirmed by PCR. |
| Type Strain： | no |
| Comments： | Type B
The presence of cpa, cpb, etx, cpb2, and pfoA genes was confirmed by PCR (assay 2, 3).
Produces hemolysins, lethal toxins, phospholipase C, and lecithinase C. In 24 to 48 hours, growth is evident by turbidity and gas formation in the broth and by colonies on the anaerobic agar surfaces. xa0No growth will occur on agar plate incubated aerobically. |
| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Anaerobic |
| Name of Depositor： | IC Hall |
| References： | Mollby R, Holme T. Production of phospholipase C (alpha-toxin), haemolysins and lethal toxins by Clostridium perfringens types A to D. J. Gen. Microbiol. 96: 137-144, 1976. PubMed: 10344 Meer RR, Songer JG. Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens. Am. J. Vet. Res. 58(7): 702-705, 1997. PubMed: 9215442 Garmory HS, et al. Occurrence of Clostridium perfringens beta2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiol. Infect. 124(1): 61-67, 2000. PubMed: 10722131 |