Construct size (kb): 4.610000133514404

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: porin
Contains complete coding sequence?: N
Genome: Homo sapiens
Gene symbol: VDAC2
Genomic copy number: unique
Gene name: voltage-dependent anion channel 2
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.36
Cross references: DNA Seq. Acc.: L06328; EST: EST06730
Nucleotides 1-352 of the insert correspond to
nucleotides 67-418 of L06328 with 99% identity.
DNA Seq. Acc.: L06328; EST: EST06729
Nucleotides 1-233 of the insert correspond to
nucleotides 1404-1172 of L06328 with 96% identity.
DNA Seq. Acc.: T08837; EST: EST06729; Primer: M13-21; THC
id: THC96654
DNA Seq. Acc.: T08838; EST: EST06730; Primer: M13 Reverse;
THC id: THC123201

Insert lengths(kb): 1.360000014305115

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: voltage-dependent anion channel 2 VDAC2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; HindIII/NotI--3.3, 0.8, 0.56.