宁波泰斯拓生物

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p422 [p4.2.2]

货号 TS183480
中文名称 null
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产品名称: p422 p4.2.2
商品货号: TS183480
Designations: p422 p4.2.2
Species: Mus musculus, mouse
Depositors: RH Lovell-Badge
Applications:
This fragment was used to detect a 3.5 kb EcoRI male-specific band of mouse genomic DNA.
Vector:
Construct size (kb): 6.659999847412109
Insert:
DNA: genomic
Insert lengths(kb): 3.700000047683716
Tissue: spleen
Gene product: sex determining region of chromosome Y(sex determining region Y) Sry
Insert Size (kb): 3.700
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.9; HindIII--3.2, 2.4, 1.0; BglII--6.6; PstI--5.2, 1.4; XbaI--3.0, 2.0, 1.6.
A 0.38 kb BglII/PstI fragment is non-repetitive and contains sequences homologous to the probe, pY53.3, which contains the human sex-determining region. This fragment was used to detect a 3.5 kb EcoRI male-specific band of mouse genomic DNA.
Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References:

Gubbay J, et al. A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes. Nature 346: 245-250, 1990. PubMed: 2374589