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| 产品名称： | pBSL128 |
|---|---|
| 商品货号： | TS183506 |
| Designations： | pBSL128 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0599999427795410 Vector: pBSL128 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript Marker(s):ampR,kanR Construct size (kb): 5.059999942779541 Features: marker(s): ampR promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: nptII (kanR) |
| Applications： | contains easily purifiable cassette(s) for construction omega-nptII |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 2.0; EcoRI--2.9, 2.1; KpnI--2.9, 2.0; MluI--3.0, 2.0; SstI--3.0, 2.0. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA. Plasmid useful for vector construction. Contains a nptII (kanR) cassette flanked by omega elements and multiple cloning sites duplicated in inverted orientation. SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 |
| Shipped： | freeze-dried |