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微信小章| 产品名称： | Plasmodium vinckei vinckei Rhodain |
|---|---|
| 商品货号： | TS183561 |
| Application： | Vector borne research |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | mosquito, Anopheles dureni, Kinga River, Katanga, Zaire, 1952 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Not capable of developing in mosquito vectors. cellular Mechanism in the Immune Response Serial passage in white mice. Inject contents of ampule intraperitoneally into 2 or 3 white mice. |
| Growth Conditions： | Duration: in vivo, laboratory mouse |
| Cryopreservation： | CRYOPRESERVATION:xa0 Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust. 1.xa0xa0 To harvest parasites, inject host with ketamine (0.1x960.2 ml). 2.xa0xa0 Open chest cavity to expose heart and exsanguinate via cardiac puncture using Yaegers anticoagulant** (see below), 1 volume anticoagulant to 4 volumes blood.xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Glass distilled HGlaxa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 3.xa0xa0 Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube. 4.xa0xa0 Aspirate supernatant using sterile Pasteur pipet. 5.xa0xa0 Resuspend pellet gently in remaining supernatant. 6.xa0xa0 Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows: A.xa0 Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature. B.xa0 Add the remaining 4 volumes of glycerolyte and gently agitate. 7.xa0xa0 Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.). 8.xa0xa0 Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor. xa0xa0xa0xa0xa0xa0xa0 |
| Name of Depositor： | M Yoeli |
| Chain of Custody： | ATCC < |
| Year of Origin： | 1952 |
| References： | Nussenzweig RS, et al. Studies on the protective effect of Plasmodium chabaudi infection in mice upon a subsequent infection with another rodent malaria species, Plasmodium vinckei. Mil. Med. 131: 1237-1242, 1966. PubMed: 4957831 Yoeli M, Most H. A study of inapparent infections of Plasmodium vinckei in the adult hamster (Mesocricetus auratus). Trans. R. Soc. Trop. Med. Hyg. 64: 276-278, 1970. PubMed: 5449053 Wasserman GM, et al. An approach to development of specific t-lymphocyte lines by use of preprocessed antigens in Plasmodium vinckei vinckei murine malaria. Infect. Immun. 61: 1958-1963, 1993. PubMed: 8478085 Perlmann H, et al. Cellular mechanisms in the immune response to malaria in Plasmodium vinckei-infected mice. Infect. Immun. 63: 3987-3993, 1995. PubMed: 7558309 |