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| 产品名称： | pCGS966 CGE1638 |
|---|---|
| 商品货号： | TS183625 |
| Designations： | pCGS966 CGE1638 |
| Depositors： | Collaborative Research, Inc., DT Moir, Collaborative Research, Inc. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 19.5000000000000000 Vector: pCGS966 (YAC) Construction: pYAC3, pYACneo, pAYE56 Marker(s):TRP1,URA3,ampR,mtxR Construct size (kb): 19.5 Features: marker(s): ampR, TRP1, URA3, mtxR replicon: pMB1, SV40, ARS1 centromere: CEN4 |
| Applications： | YL-type (YAC) shuttle vector shuttle vector vector for constructing genomic libraries |
| Comments： | Restriction sites available for inverse PCR rescue include MboI (flanking EcoRI by 107 and 77 bp), RsaI (flanking EcoRI by 297 and 352 bp), and TaqI (flanking EcoRI by 508 and 869 bp). Restriction digests of the clone give the following sizes (kb): BamHI--18.0, 1.8; EcoRI--20.0; HindIII--5.4, 4.5, 3.3, 2.7, 1.7, 1.4. Induced transcription from a GAL1 promoter inactivates the centromere and increases copy number. Additional amplification results from growth in media containing methotrexate, sulfanilamide and thymidine. This YAC cloning vector has bacterial replication origins and selectable markers on both arms to permit rescue of end subclones from each end in Escherichia coli. This YAC cloning vector has a conditional centromere for copy number amplification of yeast artificial chromosomes, T3 and T7 promoters, restriction sites for inverse PCR rescue, and a replication origin and markers functional in mammalian cells. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Smith DR, et al. Amplification of large artificial chromosomes. Proc. Natl. Acad. Sci. USA 87: 8242-8246, 1990. PubMed: 2236036 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the YAC |