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| 产品名称： | pEcoRIB |
|---|---|
| 商品货号： | TS183717 |
| Designations： | pEcoRIB |
| GenBank Number： | N00021 |
| Species： | Rous sarcoma virus |
| Depositors： | J Bishop |
| Vector： | Construct size (kb): 7.25 |
| Insert： | DNA: genomic Insert lengths(kb): 2.950000047683716 Gene product: avian sarcoma (Schmidt-Ruppin A-2) viral (v-src) oncogene homolog SRC |
| Insert Size (kb)： | 2.950 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: Frozen E. coli containing the plasmid |
| Comments： | The insert contains HindIII, SmaI (3), PvuII (3), HIndII, and PvuI sites. Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.0; HindIII--7.4; PvuII--2.7, 2.3, 1.5, 0.9. The insert contains a 0.8 kb PvuII fragment which was subcloned into pBR322 to form pPvuIIE (ATCC 41006). Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| Classification： | Retrovirus, Avian Type C Retrovirus Group |
| References： | DeLorbe WJ, et al. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J. Virol. 36: 50-61, 1980. PubMed: 6255214 Barbara Weiffenbach, personal communication |