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| 产品名称： | pUC14A1 |
|---|---|
| 商品货号： | TS183760 |
| Designations： | pUC14A1 |
| Species： | Homo sapiens, human |
| Depositors： | L Tsui |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.5 Tissue: adult liver Gene product: crystallin, beta A1(crystallin, beta polypeptide 1) CRYB1 Alleles: A1, A2, A1, A2 |
| Insert Size (kb)： | 2.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--2.95, 2.35; EcoRI--2.8 doublet. The insert corresponds to the 3 flanking region of the gene. The probe hybridizes to two HindIII-digested human DNA fragments, a strong 2.3 kb band and a weak 4.0 kb band. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The insert is an EcoRI fragment derived from lambda14A. The 3 EcoRI site is of phage origin. Enzyme(s) not detecting polymorphism: EcoRI, BamHI, HindIII, XbaI, TaqI, MspI, RsaI, PvuII, BglI, BglII, SmaI. |
| References： | Law ML, et al. Localization of a beta-crystallin gene, HubetaA3/A1 (gene symbol: CRYB1), to the long arm of human chromosome 17. Cytogenet. Cell Genet. 42: 202-207, 1986. PubMed: 3757553 Hogg D, et al. Characterization of the human beta-crystallin gene HubetaA3/A1 reveals ancestral relationships among the betagamma-crystallin superfamily. J. Biol. Chem. 261: 12420-12427, 1986. PubMed: 3745196 Barbara Weiffenbach, personal communication |