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| 产品名称： | pCI-Rat-NFL |
|---|---|
| 商品货号： | TS184027 |
| Designations： | pCI-Rat-NFL |
| GenBank Number： | AF031880 |
| Species： | Rattus norvegicus, rat |
| Depositors： | RK Liem |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: brain, 21-day-old Gene product: neurofilament, light polypeptide (68kD)( neurofilament light protein, neurofilament protein, light polypeptide) Nefl |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--6.0; EcoRI--3.4, 1.75;EcoRI/NotI--3.5, 1.8. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.05; BglII--0.80; KpnI--1.17; PvuI--0.37; PvuII--0.35, 1.07; SacI--1.32; SmaI--0.04, 0.14; XbaI--1.06; XhoI--1.38; XhoII--0.80. |
| References： | Chin SS, Liem RK. Expression of rat neurofilament proteins NF-L and NF-M in transfected non-neuronal cells. Eur. J. Cell Biol. 50: 475-490, 1989. PubMed: 2516804 Leung CL, et al. The intermediate filament protein peripherin is the specific interaction partner of mouse BPAG1-n (Dystonin) in neurons. J. Cell Biol. 144: 435-446, 1999. PubMed: 9971739 |