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| 产品名称： | pT7/Nde |
|---|---|
| 商品货号： | TS184062 |
| Designations： | pT7/Nde |
| Depositors： | RS Haun |
| Other IDs： | Nucleotide (GenBank) : U25273 Expression cloning vector pT7/Nco, complete sequence. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.9000000953674320 Vector: pT7/Nde (plasmid) Promoters: Promoter SP6 Construction: pGEM-3, pET-3a Marker(s):ampR Construct size (kb): 2.900000095367432 Features: marker(s): ampR promoter: SP6 promoter: T7 (phi10) replicon: pMB1 MCS: HindIII...XbaI ribosome-binding site: gene 10 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--2.90; HindIII--2.90; NdeI--2.90. The NdeI site (CATATG) includes the initiator methionine codon. The NdeI cloning site can also be used in conjunction with the downstream BamHI site. Differs from pT7/NcoI (ATCC 87051) only in the cloning site and reading frame for the insert. Expression vector, maintained at high copy number. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Hong JX, et al. Effect of ADP-ribosylation factor amino-terminal deletions on its GTP-dependent stimulation of cholera toxin activity. J. Biol. Chem. 269: 9743-9745, 1994. PubMed: 8144566 |
| Shipped： | freeze-dried |