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| 产品名称： | pWE15 |
|---|---|
| 商品货号： | TS184094 |
| Designations： | pWE15 |
| Depositors： | GA Evans |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.1999998092651370 Vector: pWE15 (cosmid) Promoters: Promoter T3 Marker(s):G418R,ampR,kanR Construct size (kb): 8.199999809265137 Features: marker(s): ampR, kanR, G418R promoter: T3, T7 replicon: pMB1 |
| Applications： | contains easily purifiable cassette(s) for construction expression vector integrating vector shuttle vector vector for constructing genomic libraries vector permitting RNA synthesis in vitro vector permitting positive selection for integration |
| Comments： | pWE15 (TS184094) and pWE16 (ATCC 37524) differ only in the markers selectable in vertebrate cells. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid vector for rapid genomic walking, restriction mapping and gene transfer, with selectable markers in either Escherichia coli or mammalian cell lines. Contains promoters T3 and T7 flanking the BamHI site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Wahl GM, et al. Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. Proc. Natl. Acad. Sci. USA 84: 2160-2164, 1987. PubMed: 3031668 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |