微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pFBR-1 |
|---|---|
| 商品货号： | TS184099 |
| Designations： | pFBR-1 |
| Species： | Murine osteosarcoma virus |
| Depositors： | T Curran |
| Applications： | in another host, produces protein p75 gag-fos |
| Vector： | Construct size (kb): 12.0 |
| Insert： | DNA: genomic Insert lengths(kb): 7.699999809265137 Tissue: transformed Fischer rat embryo 208F cell line Gene product: p75 gag-fos gag-fos Target Gene: p75 gag-fos |
| Insert Size (kb)： | 7.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): HindIII--7.7, 4.4; XhoI--12.1; BamHI--6.2, 4.7, 1.3. Ap(r). Virus clone biologically active on DNA transfection, rescued virus induces osteosarcomas. Infected D55 cells obtained from ATCC. Constructed by inserting EcoRI digested FBR-MSU integrated pro-viral DNA from non-producer cells Ap(r). Virus clone biologically active on DNA transfection, rescued virus induces osteosarcomas. Biologically active; upon transfection rescued virus induces osteosarcomas. The p75 gag-fos fusion protein is a nuclear phosphoprotein, antigenically related to p12gag, p15gag and p30gag as well as v-fos. This clone was constructed by inserting EcoRI-digested FBR-MSV integrated proviral DNA from non-producer cells into the EcoRI site of Charon 30; the HindIII fragment was then subcloned into the HindIII of pBR322. |
| References： | Curran T, Verma IM. FBR murine osteosarcoma virus. 1. Molecular analysis and characterization of a 75,000-Da gag-fos fusion product. Virology 135: 218-228, 1984. PubMed: 6203214 Van Beveren C, et al. FBR murine osteosarcoma virus. II. Nucleotide sequence of the provirus reveals that the genome contains sequences acquired from two cellular genes. Virology 135: 229-243, 1984. PubMed: 6203215 |