微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pRB374 |
|---|---|
| 商品货号： | TS184103 |
| Designations： | pRB374 |
| Depositors： | R Bruckner |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Intact vector size: 5.900
Type of vector: plasmid Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI PstI SphI HindIII Construction: pUB110, pBR322, vegII Host range: Bacillus subtilis; Escherichia coli Features (with orientation and position when available): terminator: To phage lambda promoter: vegII (B. subtilis) MCS: HindIII...EcoRI transcription terminator: rrnB marker(s): ampR replicon: pMB1 replicon: pUB110 marker(s): neoR marker(s): bleR Vector: pRB374 (plasmid)
Promoters: Promoter vegII (B. subtilis)
Construction: pUB110, pBR322, vegII
Marker(s): ampR,bleR,neoR
Construct size (kb): 5.900
Features: marker(s): ampR marker(s): bleR marker(s): neoR promoter: vegII (B. subtilis) replicon: pMB1 replicon: pUB110 MCS: HindIII...EcoRI terminator: To phage lambda transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.9; BamHI--5.9; BglI/BglII--3.5, 2.4. The Bacillus subtilis promoter vegII initiates transcription in both B. subtilis and E. coli. Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. May not be suitable for cloning very strong expression signals. Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028 |
| Related Products： | Component of: ATCC 87056 |
| Shipped： | freeze-dried |