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| 产品名称： | pMCT32.1 RW-148 |
|---|---|
| 商品货号： | TS184108 |
| Designations： | pMCT32.1 RW-148 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Subcloned from cMCT32; RFLPs observed under normal stringency (0.1X SSC). |
| Vector： | Construct size (kb): 5.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Gene product: DNA Segment, single copy D3S31 Alleles: B2, B1, A1, A2, A3, C2, C1 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--6.2; EcoRI--5.1, 0.79; HindIII/EcoRI--2.75, 2.3, 0.79; HindIII, BamHI--2.75, 2.45, 0.61; SalI--uncut. Codominant segregation of the PvuII RFLP has been demonstrated in 29 3-generation families. Subcloned from cMCT32; RFLPs observed under normal stringency (0.1X SSC). Enzyme(s) not detecting polymorphism: RsaI, PstI, BglII, EcoRI, BamHI, HindIII. |
| References： | Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence (pMCT32.1) on chromosome 3 D3S31. Nucleic Acids Res. 16: 9358, 1988. PubMed: 2902585 |