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| 产品名称： | pT1.11 |
|---|---|
| 商品货号： | TS184140 |
| Designations： | pT1.11 |
| GenBank Number： | M24843 |
| Species： | Mus musculus, mouse |
| Depositors： | R Klemenz |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.70199990272522 Tissue: NIH 3T3 cell line Gene product: T1 protein, carcinoembryonic antigen-related T1* |
| Insert Size (kb)： | 2.702 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--3.0, 2.7; BglI--3.2, 1.3, 1.15; EcoRI--2.9, 1.7, 1.1; PstI--3.3, 2.4; PvuII--2.5, 1.6, 1.5. The insert includes the following restriction sites (approximate kb from the 5 end): EcoRI--1.0; PvuII--1.22; BglI--1.8; KpnI--2.0; PstI--2.4. Contains the complete coding sequence (nt 192-1205) and 5 and 3 untranslated regions. The 5 end of the cDNA is 16 bp downstream of the transcription initiation site. Transcription from the T3 promoter gives the sense strand. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Klemenz R, et al. Serum- and oncoprotein-mediated induction of a gene with sequence similarity to the gene encoding carcinoembryonic antigen. Proc. Natl. Acad. Sci. USA 86: 5708-5712, 1989. PubMed: 2527364 Roman Klemenz, personal communication |