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微信小章| 产品名称： | PHM1-41 |
|---|---|
| 商品货号： | TS184181 |
| Organism： | Homo sapiens, human |
| Tissue： | uterine myometrium smooth muscle |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0cells contain Human Papilloma (HPV-16) viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The PHM1-41 cell line was derived from pregnant human myometrium obtained at time of elective cesarean section, 39 weeks gestation, patient was not in labor, no complications in the pregnancy. xa0 Myometrial cells were isolated by enzymatic digestion, infected at passage 2 with replication-defective adenovirus vector pLXSN16E6E7 expressing the E6/E7 proteins of human papilloma virus 16 and the neomycin resistance gene, and selected with the neomycin analog Geneticin. The designation -41 represent the subclones of the original immortalization mixture, selected by cloning rings for smooth muscle morphology but were not derived by limiting dilution cloning. |
| Clinical Data： | female patient, pregnant myometrium (39 weeks gestation) |
| Comments： | PHM1-41 cells retain many morphological and phenotypic responses in common with cultured primary myometrial cells. xa0They are useful for long-term or coordinated studies,as their behavior is quite reproducible and the alternative, obtaining material from patients, is labor intensive and variable. xa0These cells have been used to study crosstalk signaling between contractant and relaxant pathways, oxytocin receptor signaling pathways, and signal regulated control of calcium dynamics in the cytosol and endoplasmic reticulum compartments. xa0Furthermore the can be used for studies on ion channel activity, expression, and gene regulation. xa0 PHM1-41 does not grow in soft agar. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, (ATCC® No. 30-2002). To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended. Medium renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95% supplemented with heat-inactivated fetal bovine serum + 5% DMSO |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | TH01: 7 D5S818: 11, 13 D13S317: 14 D7S820: 8, 10 D16S539: 10, 12 CSF1PO: 11 Amelogenin: X vWA: 16, 17 TPOX: xa08, 11 |
| Sterility Tests： | Pass |
| Passage Number： | 12 |
| Population Doubling Time： | 43.2 hours |
| Name of Depositor： | B Sanborn |
| Year of Origin： | 1992 |
| References： | Monga M, et al. Oxytocin-stimulated responses in a pregnant human immortalized myometrial cell line. Biol. Reprod. 55(2): 427-32, 1996. PubMed: 8828850 Yang M, et al. Multiple Trp isoforms implicated in capacitative calcium entry are expressed in human pregnant myometrium and myometrial cells. Biol Reprod 67(3):988-94, 2002. PubMed: 12193412 Zhong M., et al. Extracellular signal-regulated kinase 1/2 activation by myometrial oxytocin receptor involves Galpha(q)Gbetagamma and epidermal growth factor receptor tyrosine kinase activation. Endocrinology 144(7): 2947-56, 2003. PubMed: 12810550 Zhong M., et al. Multiple signals regulate phospholipase CBeta3 in human myometrial cells. Biol Reprod. 78(6): 1007-17, 2008. PubMed: 18322273 Murtazina DA, TRPC1, STIM1, and ORAI influence signal-regulated intracellular and endoplasmic reticulum calcium dynamics in human myometrial cells. Biol. Reprod. 85(2): 315-26, 2011. PubMed: 21565997 |