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微信小章| 产品名称： | Murid herpesvirus 4 |
|---|---|
| 商品货号： | TS184196 |
| Classification： | Herpesviridae, Rhadinovirus |
| Deposited As： | Murine gammaherpesvirus 68 |
| Agent： | Murid herpesvirus 4 |
| Strain： | WUMS |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | GenBank: U97553 |
| Effect on Host： | CPE, cell rounding and clustering |
| Recommended Host： | BALB/3T12-3 (ATCC® CCL-164™) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-10 days |
| Name of Depositor： | KE Weck, University of Washington School of Medicine |
| Special Collection： | NCRR Contract |
| Source： | Pool of lung, spleen, heart, liver, kidney from bank vole (Clethrionomys glareolus), Czechoslovakia, 1976 |
| Geographical Isolation： | Bratislava area |
| References： | Blaskovic D, et al. Isolation of five strains of herpesviruses from two species of free living small rodents. Acta Virol. 24: 468, 1980. PubMed: 6111210 Efstathiou S, et al. Cloning and molecular characterization of the murine herpesvirus 68 genome. J. Gen. Virol. 71: 1355-1364, 1990. Mackett M, et al. Genetic content and preliminary transcriptional analysis of a representative region of murine gammaherpesvirus 68. J. Gen. Virol. 78: 1425-1433, 1997. PubMed: 9191940 Virgin HW, et al. Complete sequence and genomic analysis of murine gammaherpesvirus 68. J. Virol. 71: 5894-5904, 1997. PubMed: 9223479 Weck KE, et al. Murine gamma-herpesvirus 68 causes severe large-vessel arteritis in mice lacking interferon-gamma responsiveness: a new model for virus-induced vascular disease. Nat. Med. 3: 1346-1353, 1997. PubMed: 9396604 |
| Cross References： | Nucleotide (GenBank) : U97553 Murine herpesvirus 68 strain WUMS, complete genome |