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| 产品名称： | pHUGP13 pGEM4-HUGP-13 |
|---|---|
| 商品货号： | TS184206 |
| Designations： | pHUGP13 pGEM4-HUGP-13 |
| GenBank Number： | M15182 |
| Species： | Homo sapiens, human |
| Depositors： | WS Sly |
| Vector： | Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-4 Intact vector size: 2.870 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): MCS: EcoRI...HindIII, -> promoter for in vitro transcription: T7, replicon: pMB1 marker(s): ampR, promoter for in vitro transcription: SP6, -> Cross references: DNA Seq. Acc.: X65303 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GUSB Genomic copy number: unique Gene name: glucuronidase, beta Contains complete coding sequence?: Y Chromosome: 7; Localization: 7 q22 Tissue: placenta Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 2.191 Cross references: DNA Seq. Acc.: M15182 Nucleotides 1-2191 of the insert correspond to nucleotides 1-2191 of M15182. Insert lengths(kb): 2.190999984741211 Tissue: placenta Gene product: glucuronidase, beta(beta-glucuronidase) GUSB |
| Insert Size (kb)： | 2.191 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.5, 1.1, 0.72; BamHI/HindIII--3.5, 1.1, 0.72; EcoRI--2.9, 2.3; HindIII--5.2; PstI--3.6, 0.88; 0.62. The insert contains the following restriction sites (approximate kb from the 5 end): NotI--0.2; PstI--0.1, 0.67, 1.58; BamHI--1.0, 1.68. |
| References： | Oshima A, et al. Cloning, sequencing, and expression of cDNA for human beta-glucuronidase. Proc. Natl. Acad. Sci. USA 84: 685-689, 1987. PubMed: 3468507 |