宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pTK-HSV-SCAP-T7

货号 TS184211
中文名称 null
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产品名称: pTK-HSV-SCAP-T7
商品货号: TS184211
Designations: pTK-HSV-SCAP-T7
GenBank Number:

U67060

Species: Cricetus cricetus, hamster
Depositors: JL Goldstein
Applications:
in another host, produces protein SREBP cleavage-activating protein
Vector:

Name of construct: pTK-HSV-SCAP-T7
Excise insert: ClaI+NdeI
Promoter giving sense strand: TK
Size of construct (kb): 10.0
Markers:
ampR, G418R

Name of vector: pTK-HSV/T7
Intact vector size: 6.0
Type of vector: phagemid
Vector end: ClaI
Vector end: NdeI
Cloning sites: ClaI NdeI
Cloning capacity:
pcDNA3, TK, HSV
Host range: mammalian cells; Escherichia coli

Features (with orientation and position when available):
promoter for expression: TK, ->
restriction site: NotI
promoter for in vitro transcription: T7, ->
stop codons: TAG, ->
restriction site: SpeI NheI
initiation codon: ATG, ->
epitope tag: HSV, ->
restriction site: ClaI
restriction site: NdeI
epitope tag: T7, ->
stop codons: TGA, ->
promoter for in vitro transcription: SP6, <-
terminator: bGH polyadenylation, ->
replicon: f1, ->
replicon: SV40, ->
promoter: SV40, ->
marker(s): G418R, ->
terminator: SV40 polyadenylation, ->
replicon: pMB1
marker(s): ampR, <-

Insert:
Genome: hamster
Gene symbol: SCAP*
Gene product: SREBP cleavage-activating protein SCAP*
Contains complete coding sequence?: N
Type of DNA: cDNA
Insert size (kb): 3.8
Cross references: DNA Seq. Acc.: U67060
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed in Escherichia coli HB101 (ATCC 33694)
Comments:
Restriction digests of the clone give the following sizes (kb): ClaI--10.0; ClaI/NdeI--6.0, 4.0; NdeI--10.0.
Insert encodes amino acids 2-1276.
References:

Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195

Hua X, et al. Regulated cleavage of sterol regulatory element binding proteins requires sequences on both sides of the endoplasmid reticulum membrane. J. Biol. Chem. 271: 10379-10384, 1996. PubMed: 8626610

Joseph L Goldstein, personal communication