| 产品名称: | pTK-HSV-SCAP-T7 |
|---|---|
| 商品货号: | TS184211 |
| Designations: | pTK-HSV-SCAP-T7 |
| GenBank Number: | U67060 |
| Species: | Cricetus cricetus, hamster |
| Depositors: | JL Goldstein |
| Applications: | in another host, produces protein SREBP cleavage-activating protein |
| Vector: | Name of construct: pTK-HSV-SCAP-T7 Name of vector: pTK-HSV/T7 |
| Insert: | Genome: hamster Gene symbol: SCAP* Gene product: SREBP cleavage-activating protein SCAP* Contains complete coding sequence?: N Type of DNA: cDNA Insert size (kb): 3.8 Cross references: DNA Seq. Acc.: U67060 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed in Escherichia coli HB101 (ATCC 33694) |
| Comments: | Restriction digests of the clone give the following sizes (kb): ClaI--10.0; ClaI/NdeI--6.0, 4.0; NdeI--10.0. Insert encodes amino acids 2-1276. |
| References: | Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195 Hua X, et al. Regulated cleavage of sterol regulatory element binding proteins requires sequences on both sides of the endoplasmid reticulum membrane. J. Biol. Chem. 271: 10379-10384, 1996. PubMed: 8626610 Joseph L Goldstein, personal communication |