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| 产品名称： | pBSL193 |
|---|---|
| 商品货号： | TS184238 |
| Designations： | pBSL193 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.2750000953674320 Vector: pBSL193 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript Marker(s):ampR,tetR Construct size (kb): 5.275000095367432 Features: marker(s): ampR promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: tetR |
| Applications： | contains easily purifiable cassette(s) for construction omega-tet |
| Comments： | Restriction digests of the clone give the following sizes (kb):BamHI--3.0, 2.3; EcoRI--3.0, 1.45, 0.6, 0.4; KpnI--3.0, 2.3; MluI--3.15, 2.2; SstI--3.0, 2.35. Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Tc element flanked by multiple cloning sites duplicated in tandem orientation. Corresponds to the EcoRI/StyI fragment of pBR322, with BamHI, SalI, AccI, HincII, and HindIII sites removed. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 |
| Shipped： | frozen |