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| 产品名称： | pAS2 pAS1-CYH2 |
|---|---|
| 商品货号： | TS184359 |
| Designations： | pAS2 pAS1-CYH2 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.5000000000000000 Vector: pAS2 (phagemid) Promoters: Promoter ADC1 (ADH) Construction: pAS1 Marker(s):TRP1,ampR,cyhS Features: marker(s): CYH2 marker(s): TRP1 marker(s): ampR other: GAL4 DNA-binding domain promoter: ADC1 (ADH) replicon: 2 micron replicon: f1+ replicon: pMB1 MCS: NdeI...SalI terminator: ADC1 epitope tag: hemagglutinin (HA) |
| Applications： | YE-type (episomal) shuttle vector contains sequence ribosomal protein L29 Ribosomal protein RL43 expression vector shuttle vector vector for constructing cDNA libraries vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.6; SalI--8.6; SmaI--8.6. Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |