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| 产品名称： | P4D0102 |
|---|---|
| 商品货号： | TS184361 |
| Designations： | P4D0102 |
| Depositors： | FM Ausubel |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.1999998092651400 Vector: P4D0102 (plasmid) Construction: P4D0100, alphaam52 Marker(s):tetR Construct size (kb): 10.19999980926514 Features: marker(s): tetR replicon: pMB1, P4 |
| Applications： | shuttle vector |
| Comments： | Vector maintained either as a self-replicating plasmid or as an integrated prophage, depending on the presence of a tRNA nonsense-codon suppressor in the host strain. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ow DW, Ausubel FM. Conditionally replicating plasmid vectors that can integrate into the Klebsiella pneumoniae chromosome via bacteriophage P4 site-specific recombination. J. Bacteriol. 155: 704-713, 1983. PubMed: 6307977 |
| Shipped： | freeze-dried |