微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pRK65-6 |
|---|---|
| 商品货号： | TS184386 |
| Designations： | pRK65-6 |
| GenBank Number： | X16563 |
| Species： | Rattus norvegicus, rat |
| Depositors： | JR Sanes |
| Applications： | produces protein laminin, S polypeptide (synapses) laminin, S polypeptide |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.5 Tissue: kidney Gene product: laminin, S polypeptide (synapses)(laminin, S polypeptide) LAMS Target Gene: laminin, S polypeptide (synapses) |
| Insert Size (kb)： | 2.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.4, 0.72, 0.56, 0.45, 0.31; EcoRI--3.2, 2.4; HindIII--5.0, 0.58; AvaI--3.6, 1.8, 0.31; BamHI--5.7. The insert encodes the carboxy-terminal 40% of s-laminin. A fusion protein product is recognized by the monoclonal antibodies C4, D5, and D7. This clone detects a mRNA of about 5.7 kb in rat kidney and muscle. |
| References： | Hunter DD, et al. Primary sequence of a motor neuron-selective adhesive site in the synaptic basal lamina protein s-laminin. Cell 59: 905-913, 1989. PubMed: 2590946 Hunter DD, et al. A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction. Nature 338: 229-233, 1989. PubMed: 2922051 |