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| 产品名称： | lambdaADH |
|---|---|
| 商品货号： | TS184399 |
| Designations： | lambdaADH |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 42.40 DESCRIPTION OF VECTOR: Intact vector size: 42.400 Type of vector: phage Cloning sites: XhoI EcoRI Polylinker sites: BglII EcoRI BamHI XhoI BglII Cloning capacity: 0 - 8.4 kb Construction: lambdaYES-R, pADH Host range: Escherichia coli Features (with orientation and position when available): other: left arm (19.6 kb), -> other: lox, -> replicon: ARS1 replicon: pMB1 marker(s): ampR, promoter for expression: ADH, -> MCS: XhoI EcoRI, -> promoter for expression: lac, transcription terminator: HIS3, -> marker(s): URA3, -> centromere: CEN4 other: right arm (15.2 kb, with cI857) Vector: lambdaADH (phage, lambda - replacement) Promoters: Promoter for expression ADH Construction: lambdaYES-R, pADH Marker(s):URA3,ampR Construct size (kb): 42.40 Features: marker(s): URA3 marker(s): ampR other: left arm (19.6 kb) other: lox other: right arm (15.2 kb, with cI857) promoter for expression: ADH promoter for expression: lac replicon: ARS1 replicon: pMB1 MCS: XhoI EcoRI transcription terminator: HIS3 centromere: CEN4 |
| Applications： | expression vector lambda vector allowing conversion to plasmid vector for constructing cDNA libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--43.0; SalI--21.0, 13.5, 13.0; XhoI--25.0, 21.0. Transcription initiated from the ADH promoter in yeast hosts can be terminated at the HIS3 terminators. Vector for the construction of cDNA libraries permitting regulated expression in both yeast and E. coli and simple conversion to plasmid via the cre-lox system. The plasmid derivative (pADH, 7.75 kb) is a YC-type shuttle expression vector. The lac promoter contains a ribosome binding sequence optimally spaced from an AUG start codon and can support translational fusions. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stephen J Elledge, personal communication |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |