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| 产品名称： | pHRL83-IVS4 |
|---|---|
| 商品货号： | TS184404 |
| Designations： | pHRL83-IVS4 |
| Species： | Homo sapiens, human |
| Depositors： | LH Kedes |
| Vector： | Construct size (kb): 4.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 0.4000000059604645 Gene product: actin, alpha, cardiac muscle ACTC |
| Insert Size (kb)： | 0.400 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; HindIII--4.8; BamHI--4.8; PstI--4.4, 0.4. This probe hybridizes to human but not rodent cardiac actin sequences under the following conditions: 4X SSC; 50 mM NaH2P04; 5 X Denhardts, 10% dextran sulfate at 65C. Wash at 0.5X SSC, 0.1% SDS, 65 C. The insert corresponds to intervening sequence 4 of the gene. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References： | Gunning P, et al. Expression of human cardiac actin in mouse L cells: A sarcomeric actin associates with a nonmuscle cytoskeleton. Cell 36: 709-715, 1984. PubMed: 6538118 Gunning P, et al. Chromosomal location of the co-expressed human skeletal and cardiac actin genes. Proc. Natl. Acad. Sci. USA 81: 1813-1817, 1984. PubMed: 6584914 Barbara Weiffenbach, personal communication |