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| 产品名称： | pBT142 |
|---|---|
| 商品货号： | TS184451 |
| Designations： | pBT142 |
| Depositors： | JC Alonso |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.6599998474121090 Vector: pBT142 (plasmid) Promoters: Promoter none Construction: pUB110, pNM481 Marker(s):bleR,kanR,neoR Construct size (kb): 6.659999847412109 Features: insert detection: lacZ marker(s): kanR, neoR, bleR promoter: none replicon: pUB110 |
| Applications： | shuttle vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.6; SmaI--6.6; BamHI--6.6; SalI--6.6; PstI--6.6. bleR = bleomycin-pheomycin resistance One of a series (ATCC 37637 - 37639) of 3 plasmid vectors for characterization of regulatory systems in Bacillus subtilis. Inserted genes can be fused to Escherichia coli lacZ, with one vector in the series for each of the 3 translational reading frames. A 3.1 kb EcoRI/DraI fragment of pNM481 was ligated to a 3.5 kb EcoRI/PvuII fragment of pUB110. |
| Media： | ATCC® Medium 1179: VY medium |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Alonso JC. New plasmid vectors for the construction of translational gene fusions in bacillus subtilis. Gene 65: 325-328, 1988. PubMed: 3137123 |
| Shipped： | freeze-dried |