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| 产品名称： | plambda101 |
|---|---|
| 商品货号： | TS184490 |
| Designations： | plambda101 |
| Species： | Homo sapiens, human |
| Depositors： | PI Patel, C Caskey |
| Vector： | Construct size (kb): 4.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: hypoxanthine phosphoribosyltransferase pseudogene 1 HPRTP1 Alleles: A1, A2 |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--4.9; BamHI--4.9; EcoRI--4.9; PstI--3.8, 1.1; EcoRI/BamHI--2.7, 2.2. The 850 bp HaeIII fragment is free of repeated sequences. PvuII digests revealed a 5.7 kb fragment with a polymorphic 5.2 kb fragment in 1/33 chromosomes studied. These RFLPs are also seen with human HPRT cDNA but the picture is more complex because of numerous bands. The variant HindIII allele segregates independently of the variant PvuII allele. The 850 bp HaeIII fragment detects the following genomic fragments (kb): EcoRI--8.8; HindIII--9.8; BamHI--3.4 kb. This clone was derived from Hulambda10. Enzyme(s) not detecting polymorphism: TaqI, MspI, PstI, BstNI, XbaI, SacI, HincII, StuII, MboI, EcoRV. |
| References： | Patel PI, et al. Organization of the HPRT gene and related sequences in the human genome. Somatic Cell Mol. Genet. 10: 483-493, 1984. PubMed: 6089358 Dobrovic A, et al. A HindIII RFLP for the HPRT pseudogene on chromosome 3 (HPRTP1). Nucleic Acids Res. 15: 1346, 1987. PubMed: 2881273 Pragna I Patel, personal communication |