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微信小章| 产品名称： | pCMV5-REP2 |
|---|---|
| 商品货号： | TS184534 |
| Designations： | pCMV5-REP2 |
| GenBank Number： | X64728 |
| Species： | Homo sapiens, human |
| Depositors： | MC Seabra |
| Vector： | Construct size (kb): 7.599999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCMV5 Intact vector size: 4.700 Type of vector: phagemid Vector end: EcoRI Vector end: PstI Cloning sites: EcoRI BamHI HindIII Polylinker sites: Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): promoter: CMV, -> terminator: hGH, -> replicon: SV40 replicon: pMB1 marker(s): ampR, replicon: f1, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CHmL Other gene symbols: REP-2; RESP2 Gene name: choroideremia-like Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 q31-qter Type of DNA: cDNA Insert 5 end: EcoRI Insert 3 end: PstI Insert size (kb): 2.9 Cross references: DNA Seq. Acc.: X64728 Nucleotides 1-2221 of the insert correspond to nucleotides 83-2303 of X64728. Insert lengths(kb): 2.900000095367432 Gene product: choroideremia-like(Rab escort protein-2) CHML |
| Insert Size (kb)： | 2.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--4.6, 1.4, 0.9, 0.55; HindIII--6.8, 0.65; EcoRI--5.4, 1.9. The insert contains the following restricition sites (approximate kb from the 5 end): HindIII--0.38, 1.02; PstI--0.44; NcoI--0.95; HincII--1.31. Recommendation for verification: PvuII; HindIII; PstI; NcoI; HincII. |
| References： | Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778 Yamamoto R, et al. Cloning and expression of a cDNA encoding human sterol carrier protein 2. Proc. Natl. Acad. Sci. USA 88: 463-467, 1991. PubMed: 1703300 Cremers FP, et al. REP-2, a Rab escort protein encoded by the choroideremia-like gene. J. Biol. Chem. 269: 2111-2117, 1994. PubMed: 8294464 Cremers FP, et al. An autosomal homologue of the choroideremia gene colocalizes with the usher syndorme type II locus on the distal part of chromosome 1q. Hum. Mol. Genet. 1: 71-75, 1992. PubMed: 1301160 |