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微信小章| 产品名称： | EDJ#22 |
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| 商品货号： | TS184593 |
| Organism： | Mus musculus, mouse |
| Tissue： | inner cell mass |
| Cell Type： | embryonic stem cell |
| Product Format： | frozen |
| Morphology： | stem cell |
| Biosafety Level： | 1 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office (2007). The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Gender： | Male |
| Strain： | 129S5/SvEvTac |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 40 XY, diploid |
| Derivation： | The 129SvEv ES cell line, EDJ 22, is derived from 129SvEV mice from Taconic. 129SvEv is a steel substrain with pigment white-bellied agouti. Taconic indirectly obtained 129/SvEvBrd and 129/SvEv-Gpilc, and crossed them to produce 129/SvETac.xa0 Inbreeding of 129/SvEvTac has been ongoing since 1992 and the substrain is now homozygous Gpilc.Ref  Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391 |
| Clinical Data： | Male |
| Comments： | This mouse ES cell line has been shown to be germline competent. 129 and the more common, 129Sv are widely used mouse strains in gene targeting experiments.xa0 The embryonic stem cells derived from 129Sv are part of the genetic background of most mutants generated using homologous recombination. Ref
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| Complete Growth Medium： | Grow ES cells in Mouse ES Cell Basal Medium (ATCC SCRR-2011) that has been supplemented with the following components: 0.1 mM 2-mercaptoethanol (Invitrogen Cat. No. 21985) 1,000 U/ml mouse leukemia inhibitory factor (LIF) (EMD Millipore Cat. No. ESG1107) 15% FBS, ES Cell Qualified (ATCC SCRR-30-2020) Complete Growth Medium for Mouse ES Cells is stable for 14 days when stored at 2°C to 8°C. This medium is formulated for use with a 5% CO2 in air atmosphere. |
| Subculturing： |
Note: To insure the highest level of viability, pre-warm media and Trypsin/EDTA to 37ºC before adding to cells. Volumes used in this protocol are for T75 flasks. Proportionally adjust the volumes for culture vessels of other sizes. A split ratio of 1:4 to 1:7 is recommended. Feeder Cell Preparation for Subcultures
Dissociation and Transfer of ES Cells
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| Cryopreservation： | Complete growth medium supplemented with an additional 10% FBS and 10% DMSO. Make immediately prior to use. Keep at 4°C. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | TJ Ley |
| Deposited As： | Mus musculus |
| Year of Origin： | 2003 |
| References： | Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999.PubMed: 10430671 Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391 Threadgill DW, et al. Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain. Mamm. Genome. 8(6):390-393 1997. PubMed: 9166580 Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865 Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 |