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| 产品名称： | pPB1 |
|---|---|
| 商品货号： | TS184730 |
| Designations： | pPB1 |
| Depositors： | DR Tolan |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.6699988842010500 Vector: pPB1 (plasmid) Promoters: Promoter trc Construction: pUC19, pKK233-2 Marker(s):ampR Construct size (kb): 2.66999888420105 Features: marker(s): ampR promoter: trc replicon: pMB1 transcription terminator: rrnB |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; HindIII--2.7; NcoI--2.7. Expression vector. Permits high copy number and production of proteins initiated at the ATG of beta-galactosidase. The order of the major features in the plasmid is: pMB1 ori - EcoRI - trc promoter - lacZ/NcoI - PstI - HindIII - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Beernink PT, Tolan DR. Construction of a high-copy "ATG vector" for expression in Escherichia coli. Protein Expr. Purif. 3: 332-336, 1992. PubMed: 1422227 |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of xa0E. coli containing the plasmid |