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微信小章| 产品名称： | Vaccinia virus |
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| 商品货号： | TS184837 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus |
| Strain： | Modified vaccinia virus Ankara (MVA) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Attenuated vaccine strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Effect on Host： | Yes, Cytopathic effect: Rounding and death of infected cells. CPE with cell rounding and cell death |
| Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: tryptose phosphate broth to a final concentration of 5% and fetal bovine serum to a final concentration of 5%.
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| Recommended Host： | Recommended Host:xa0 CEF (ATCC® # CRL-1590) Alternate Host(s): BHK-21 (ATCC® # CCL-10) |
| Growth Conditions： | Temperature: 37.0°C Duration: 2-6 days to 3-4+ CPE when inoculated onto cells at 80-90% confluence in a T-225 flask. |
| Cryopreservation： | Storage temperature: -70 C |
| Name of Depositor： | LS Wyatt, B Moss, A Mayr |
| Special Collection： | ATCC |
| Source： | Attenuated vaccine strain |
| References： | Antoine G, et al. The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses. Virology 244: 365-396, 1998. PubMed: 9601507 Sutter G, Moss B. Nonreplicating vaccinia vector efficiently expresses recombinant genes. Proc. Natl. Acad. Sci. USA 89: 10847-10851, 1992. PubMed: 1438287 Meyer H, et al. Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulence. J. Gen. Virol. 72: 1031-1038, 1991. PubMed: 2033387 Mahnel H, Mayr A. Experiences with immunization against orthopox viruses of humans and animals using vaccine strain MVA. Berlin Munch. Tierarztl. Wochenschr. 107: 253-256, 1994. Mayr A, et al. Abstammung, eigenschafter und verwendung des attenuierten vaccinia-stammes. Infection 3: 6-14, 1975. Rotz LD, et al. Vaccinia (Smallpox) Vaccine Recommendations of the Advisory Committee on Immunization Practices (ACIP), 2001. Morb. Mortal. Wkly. Rep. 50: 1-25, 2001. or colder Wyatt LS, et al. Marker rescue of the host range restriction defects of modified vaccinia virus Ankara. Virology 251: 334-342, 1998. PubMed: 9837798 MVA was isolated by serial passage of CVA (Ankara) strain in chick embryo fibroblasts (CEF) in the laboratory of Professor Anton Mayr, then given to the National Insitutes of Health, where it was plaque purified three times in CEF cells. VR-1566 was derived by limited further passage of stock received from the NIH in the SL-29 chicken embryo fibroblast cell line ATCC CRL-1590. or colder |
| Cross References： | Nucleotide (GenBank) : U94848 Vaccinia virus strain Ankara, complete genomic sequence. |