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微信小章| 产品名称： | CCRF-CEM CCRF CEM |
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| 商品货号： | TS185035 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | T lymphoblast (KRAS CRM) |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute lymphoblastic leukemia |
| Age： | 4 years juvenile |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.xa0xa0
This line may be used as a transfection host |
| Intended Use： | Certified Reference Material produced under an ISO 17034 accredited process.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 47; range = 41 to 95. Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted. |
| Clinical Data： | Caucasian female 4 years juvenile |
| Antigen Expression： | CD3; Homo sapiens, expressed CD4; Homo sapiens, expressed CD5; Homo sapiens, expressed CD7; Homo sapiens, expressed |
| Genes Expressed： | CD3; Homo sapiens, expressed ,CD5; Homo sapiens, expressed, CD7; Homo sapiens, expressed, CD4; Homo sapiens, expressed |
| Tumorigenic： | Yes |
| Effects： | Yes, in syrian hamsters Ref   Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047 |
| Comments： | Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
Certified Reference Material produced under an ISO 17034 accredited process. Item has been tested for KRAS mutation (p.G12D c.35G>A) |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 to 3 X 105 and 1 to 2 X 106 viable cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium 95%; DMSO, 5% Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 10,13 D5S818: 12,13 D7S820: 9,13 THO1: 6,7 TPOX: 8 vWA: 17,19 |
| Isoenzymes： | ADA, 1 ES-D, 1 G6PD, B GLO-I, 1 PEP-D, 1 PGD, C PGM1, 1 PGM3, 0 |
| Name of Depositor： | GE Foley |
| Deposited As： | Homo sapiens |
| Year of Origin： | November, 1964 |
| References： | Foley GE, et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965. PubMed: 14278051 Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323 Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381 Uzman BG, et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. PubMed: 5224274 Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047 Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 CCRF-CEM is a T lymphoblastoid cell line derived by G.E. Foley, et al. using cells obtained in November, 1964 from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia. |
