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| 产品名称： | pBB115 |
|---|---|
| 商品货号： | TS185050 |
| Designations： | pBB115 |
| Species： | tomato big bud phytoplasma |
| Depositors： | RE Davis |
| Vector： | Construct size (kb): 6.80 |
| Insert： | DNA: genomic Insert lengths(kb): 3.8 Gene product: DNA Segment |
| Insert Size (kb)： | 3.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.7, 3.2; BglI--7.0; PvuII--7.0. Probe for detecting mycoplasma-like DNA in infected plants. Derived from chromosomal DNA. Hybridizes with nucleic acid from the following MLOs: tomato big bud (BB), periwinkle little leaf (CN1), Italian tomato big bud (BBIta), maize bushy stunt (MBS), potato witches broom (PWB), clover phyllody (CPh), clover proliferation (CP), ash yellows (AA), Western X disease (WX), Canada X disease (CX), and unknown (CN13, CN13G, CN13W). Does not hybridize to aster yellows (AY), vinca virescence (VR), or elm yellows (EY). |
| References： | Lee IM, et al. Nonradioactive screening method for isolation of disease-specific probes to diagnose plant diseases caused by mycoplasmalike organisms. Appl. Environ. Microbiol. 56: 1471-1475, 1990. Lee IM, et al. . Phytopathology 78: 1602, 1988. |