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| 产品名称： | YCpIF6 |
|---|---|
| 商品货号： | TS185109 |
| Designations： | YCpIF6 |
| Depositors： | RW Davis, PK Foreman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.1999998092651370 Vector: YCpIF6 (plasmid) Promoters: Promoter for expression GAL1 Construction: pUN55 Marker(s):URA3,ampR Construct size (kb): 7.199999809265137 Features: marker(s): URA3 marker(s): ampR promoter for expression: GAL1 replicon: ARS1 replicon: pMB1 MCS: SalI...SacI centromere: CEN4 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--7.2; BamHI--7.2; EcoRI--7.2. Care should be taken when cloning into this vector because several stop codons occur in this reading frame. One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter. Vectors YCpIF4, YCpIF5 and YCpIF6 (ATCC 87081-87083) differ only in the reading frame of the multiple cloning site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907 |
| Related Products： | component of:ATCC 87031 |
| Shipped： | freeze-dried |