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| 产品名称： | lambdaYES-R |
|---|---|
| 商品货号： | TS185153 |
| Designations： | lambdaYES-R |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 42.6 DESCRIPTION OF VECTOR: Intact vector size: 42.600 Type of vector: phage Cloning sites: XhoI, EcoRI Polylinker sites: EcoRI XhoI EcoRI Construction: lambdagt6; pSE936 Host range: Escherichia coli Features (with orientation and position when available): other: left arm (19.6 kb), -> other: lox, -> replicon: ARS1 replicon: pMB1 marker(s): ampR, promoter for expression: GAL1, -> MCS: XhoI EcoRI, -> promoter for expression: lac, transcription terminator: HIS3, -> marker(s): URA3, -> centromere: CEN4 other: lox, -> other: right arm (15.2 kb, with cI857) Vector: lambdaYES-R (phage, lambda - replacement) Promoters: Promoter for expression GAL1 Construction: lambdagt6; pSE936 Marker(s):URA3,ampR Construct size (kb): 42.6 Features: marker(s): URA3 marker(s): ampR other: left arm (19.6 kb) other: lox other: right arm (15.2 kb, with cI857) promoter for expression: GAL1 promoter for expression: lac replicon: ARS1 replicon: pMB1 MCS: XhoI EcoRI transcription terminator: HIS3 centromere: CEN4 |
| Applications： | expression vector lambda vector allowing conversion to plasmid vector for constructing cDNA libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0; NotI--44.0; XhoI--24.0, 20.0. The plasmid derivative (pSE936, 7.8 kb) is a YC-type shuttle expression vector. Transcription initiated from the GAL1 promoter in yeast hosts can be terminated at the HIS3 terminators. GAL1 does not provide translational start signals. Vector for the construction of cDNA libraries permitting regulated expression in both yeast and E. coli and simple conversion to plasmid via the cre-lox system. The lac promoter contains a ribosome binding sequence optimally spaced from an AUG start codon and can support translational fusions. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010 Stephen J Elledge, personal communication |
| Shipped： | frozen |
| Shipping Information： | Distributed: frozen bacteria-free lysate |