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| 产品名称： | p47-8A |
|---|---|
| 商品货号： | TS185179 |
| Designations： | p47-8A |
| GenBank Number： | M25665 |
| Species： | Homo sapiens, human |
| Depositors： | TL Leto |
| Applications： | produces protein neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1) neutrophil cytosolic factor 1 |
| Vector： | Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: NCF1 Genomic copy number: unique Gene name: neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1) Contains complete coding sequence?: Y Chromosome: 7; Localization: 7 q11.23 Tissue: HL-60 cell line (promyelocytic), differentiated Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.34 Cross references: DNA Seq. Acc.: M25665 Insert lengths(kb): 1.3400000333786 Tissue: HL-60 cell line (promyelocytic), differentiated Gene product: neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1)(neutrophil cytosolic factor 1) NCF1 Target Gene: neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1) |
| Insert Size (kb)： | 1.340 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; BglII--4.1, 0.27; BamHI--3.7, 0.64; XhoI--3.5, 0.46, 0.36, 0.23; PstI--3.3, 0.6, 0.38, 0.26. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.15, 0.33; BamHI--0.62; XhoI--0.64, 0.75, 1.06; SmaI--1.05. Contains the complete coding sequence (nt 13-1185). Detects transcripts of 2.2 (faint) and 1.5 kb. |
| References： | Francke U, et al. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. PubMed: 2393022 Lomax KJ, et al. Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease. Science 245: 409-412, 1989. PubMed: 2547247 . . Science 246: 987, 1989. |