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| 产品名称： | p426 GAL1 plasmid in E. coli |
|---|---|
| 商品货号： | TS185321 |
| Designations： | p426 GAL1 plasmid in E. coli |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.417 Vector: p426 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS426 (ATCC 77107), GAL1 promoter Marker(s):URA3,ampR Construct size (kb): 6.417 Features: marker(s): URA3 marker(s): ampR promoter for expression: GAL1 replicon: 2 micron MCS: XhoI...XpeI terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.5; EcoRI--6.4; KpnI--6.4. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of xa0E. coli containing the plasmid |