| 产品名称: | pUCI-A4 pCIA4 |
|---|---|
| 商品货号: | TS185370 |
| Designations: | pUCI-A4 pCIA4 |
| GenBank Number: | X00570 |
| Species: | Homo sapiens, human |
| Depositors: | J Scott |
| Vector: | Construct size (kb): 3.0 |
| Insert: | DNA: cDNA Insert lengths(kb): 0.4199999868869782 Gene product: apolipoprotein C-I APOC1 Alleles: A2, A1 |
| Insert Size (kb): | 0.420 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): PstI--2.5, 0.4; EcoRI--3.0; HindIII--3.0; EcoRI/HindIII--2.4, 0.5; SalI/EcoRI--3.0. The insert contains the complete coding sequence plus 5 (about 56 nucleotides) and 3 (about 110 nucleotides) of non-coding sequence. There is an APOC1 pseudogene 6 kb 3 to the functional C1 gene. The insert detects two mRNA species of 580 and 560 bases. |
| References: | Knott TJ, et al. Chromosomal localization of the human apoprotein CI gene and of a polymorphic apoprotein AII gene. Biochem. Biophys. Res. Commun. 125: 299-306, 1984. PubMed: 6095840 Knott TJ, et al. Characterisation of mRNAs encoding the precursor for human apolipoprotein CI. Nucleic Acids Res. 12: 3909-3915, 1984. PubMed: 6328444 Lusis AJ, et al. Regional mapping of human chromosome 19: organization of genes for plasma lipid transport (APOC1, -C2, and -E and LDLR) and the genes C3, PEPD, and GPI. Proc. Natl. Acad. Sci. USA 83: 3929-3933, 1986. PubMed: 3459164 |