宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pUCI-A4 [pCIA4]

货号 TS185370
中文名称 null
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产品名称: pUCI-A4 pCIA4
商品货号: TS185370
Designations: pUCI-A4 pCIA4
GenBank Number:

X00570

Species: Homo sapiens, human
Depositors: J Scott
Vector:
Construct size (kb): 3.0
Insert:
DNA: cDNA
Insert lengths(kb): 0.4199999868869782
Gene product: apolipoprotein C-I APOC1
Alleles: A2, A1
Insert Size (kb): 0.420
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): PstI--2.5, 0.4; EcoRI--3.0; HindIII--3.0; EcoRI/HindIII--2.4, 0.5; SalI/EcoRI--3.0.
The insert contains the complete coding sequence plus 5 (about 56 nucleotides) and 3 (about 110 nucleotides) of non-coding sequence.
There is an APOC1 pseudogene 6 kb 3 to the functional C1 gene.
The insert detects two mRNA species of 580 and 560 bases.
References:

Knott TJ, et al. Chromosomal localization of the human apoprotein CI gene and of a polymorphic apoprotein AII gene. Biochem. Biophys. Res. Commun. 125: 299-306, 1984. PubMed: 6095840

Knott TJ, et al. Characterisation of mRNAs encoding the precursor for human apolipoprotein CI. Nucleic Acids Res. 12: 3909-3915, 1984. PubMed: 6328444

Lusis AJ, et al. Regional mapping of human chromosome 19: organization of genes for plasma lipid transport (APOC1, -C2, and -E and LDLR) and the genes C3, PEPD, and GPI. Proc. Natl. Acad. Sci. USA 83: 3929-3933, 1986. PubMed: 3459164