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| 产品名称： | pB4 |
|---|---|
| 商品货号： | TS185439 |
| Designations： | pB4 |
| GenBank Number： | M13994 |
| Species： | Homo sapiens, human |
| Depositors： | CM Croce |
| Vector： | Construct size (kb): 3.609999895095825 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: EcoRI...HindIII, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: X02514 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: BCL2 Genomic copy number: unique Gene name: B cell CLL/lymphoma 2 Contains complete coding sequence?: U Chromosome: 18; Localization: 18 q21.3 Tissue: cell line 380 Type of DNA: cDNA Insert end: EcoRI Modification: EcoRI linkers Insert end: EcoRI Modification: EcoRI linkers Insert size (kb): 0.91 Cross references: DNA Seq. Acc.: M13994 Insert lengths(kb): 0.9100000262260437 Tissue: cell line 380 Gene product: B cell CLL/lymphoma 2 BCL2 |
| Insert Size (kb)： | 0.910 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.65, 1.0; BglI--1.9, 1.2, 0.54; PstI--3.0, 0.62; BamHI--3.3, 0.38; XbaI--3.7. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.4; PstI--0.3; PvuII--0.45; SacI--0.5; BamHI--0.65. Contains approximately nt 1410 to 2340 of the published sequence. |
| References： | Reed JC, et al. BCL2-mediated tumorigenicity of a human T-lymphoid cell line: synergy with MYC and inhibition by BCL2 antisense. Proc. Natl. Acad. Sci. USA 87: 3660-3664, 1990. PubMed: 1692620 Tsujimoto Y, Croce CM. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc. Natl. Acad. Sci. USA 83: 5214-5218, 1986. PubMed: 3523487 |