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| 产品名称： | mp40 |
|---|---|
| 商品货号： | TS185498 |
| Designations： | mp40 |
| GenBank Number： | M86671 |
| Species： | Mus musculus, mouse |
| Depositors： | U Gubler |
| Vector： | Construct size (kb): 4.013999938964844 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK+ Intact vector size: 2.964 Type of vector: phagemid Vector end: HindIII Vector end: EcoRI Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: mouse Gene symbol: Il12b Genomic copy number: unique Gene name: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) Contains complete coding sequence?: Y Chromosome: 11 Strain: C57BL/6 Tissue: spleen Type of DNA: cDNA Insert 5 end: HindIII Insert 3 end: EcoRI Insert size (kb): 1.05 Cross references: DNA Seq. Acc.: M86671 Insert lengths(kb): 1.049999952316284 Tissue: spleen Gene product: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) Il12b |
| Insert Size (kb)： | 1.050 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 1.1; BamHI--4.3; XbaI--3.2, 0.84, 0.3. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.55, 1.02; AvaI--0.52; PvuII--0.79. The sequence surrounding the initiation codon was modified to 5-ACCAUGG-3 (Kozak consensus) to optimize translation. Thus the sequence between the vector insertion site and the initiating ATG is not derived from the mRNA. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Schoenhaut DS, et al. Cloning and expression of murine IL-12. J. Immunol. 148: 3433-3440, 1992. PubMed: 1350290 |