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| 产品名称： | 42TA |
|---|---|
| 商品货号： | TS185500 |
| Organism： | Ictalurus punctatus, channel catfish |
| Tissue： | peripheral blood |
| Cell Type： | macrophage |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | diploid |
| Images： |  |
| Derivation： | The 42TA cell line was developed in 1992.The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. |
| Comments： | The 42TA cell line was developed in 1992.The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. |
| Complete Growth Medium： | A 1:1 mixture of Leibovitzs L-15 medium with 2 mM L-glutamine and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 10% double distilled water and 5% heat-inactivated catfish serum. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml. Attached cells may be subcultured by scraping. |
| Cryopreservation： | Freeze medium: Fetal bovine serum supplemented with 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 27.0°C |
| Name of Depositor： | NW Miller |
| Deposited As： | Ictalurus punctatus |
| Year of Origin： | 1992 |
| References： | Miller N, et al. Functional and molecular characterization of teleost leukocytes. Immunol. Rev. 166: 187-197, 1998. PubMed: 9914913 Miller NW, et al. Development of leukocyte cell lines from the channel catfish (Ictalurus punctatus). J. Tissue Culture Methods 16: 117-123, 1994. |