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微信小章| 产品名称： | cDMD 2b-3 30-2, cDMD2b-3 |
|---|---|
| 商品货号： | TS185510 |
| Designations： | cDMD 2b-3 30-2, cDMD2b-3 |
| GenBank Number： | M18533 |
| Species： | Homo sapiens, human |
| Depositors： | LM Kunkel |
| Applications： | For plasmid amplification, use a lac Iq host. |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.149999976158142 Tissue: fetal muscle Gene product: dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272 DMD Alleles: U2, A1, A2, C1, T1, U1, A1, A1, A2, A2, B1, B2, C2, D2, R1, R2, T1, T2, T2, T3, D1 |
| Insert Size (kb)： | 1.150 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--2.8, 1.2; BglI--2.5, 1.5; XbaI--4.3. For plasmid amplification, use a lac Iq host. Inserted into EcoRI site with 5 end closer to T7 promoter. Contains internal BglII (2) and XbaI sites. TS185510 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for TS185510, but is for ATCC 57669. Includes probes 2b and 3. Detects genomic HindIII fragments of 10.5, 4.2, 6.6, 2.7, 6, 1.7, 12, 3, and 7.3 kb. Of the DMD cDNA clones, 1-2a includes nucleotides 1-1538; 2b-3: 1455 to approximately 2600; 4-5a: 2600-4550; 5b-7: 4400-6900; 8: 6900-7800; 9-14: 7800-13900 (in base pairs from the 5 end of the cDNA). |
| References： | Lindlof M, et al. Gene deletions in X-linked muscular dystrophy. Am. J. Hum. Genet. 44: 496-503, 1989. PubMed: 2929594 Den Dunnen JT, et al. Topography of the Duchenne muscular dystrophy (DMD) gene: FIGE and cDNA analysis of 194 cases reveals 115 deletions and 13 duplications. Am. J. Hum. Genet. 45: 835-847, 1989. PubMed: 2573997 Lucotte G, et al. Molecular deletion patterns in Duchenne muscular dystrophy patients. Ann. Genet. 32: 214-219, 1989. PubMed: 2610487 Dominguez-Steglich M, et al. The dystrophin gene is autosomally located on a microchromosome in chicken. Genomics 8: 536-540, 1990. PubMed: 2286374 Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877 Koenig M, et al. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell 53: 219-228, 1988. PubMed: 3282674 Louis M Kunkel, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |